High-Resolution Typing of Leptospira interrogans Strains by Multispacer Sequence Typing
نویسندگان
چکیده
منابع مشابه
High-resolution typing of Leptospira interrogans strains by multispacer sequence typing.
Leptospirosis is a worldwide zoonosis which is responsible for the typical form of Weil's disease. The epidemiological surveillance of the Leptospira species agent is important for host prevalence control. Although the genotyping methods have progressed, the identification of some serovars remains ambiguous. We investigated the multispacer sequence typing (MST) method for genotyping strains bel...
متن کاملMultilocus sequence analysis for typing Leptospira interrogans and Leptospira kirschneri.
Fifty-three strains belonging to the pathogenic species Leptospira interrogans and Leptospira kirschneri were analyzed by multilocus sequence analysis. The species formed two distinct branches. In the L. interrogans branch, the phylogenetic tree clustered the strains into three subgroups. Genogroups and serogroups were superimposed but not strictly.
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Bartonella quintana is a worldwide fastidious bacterium of the Alphaproteobacteria responsible for bacillary angiomatosis, trench fever, chronic lymphadenopathy, and culture-negative endocarditis. The recent genome sequencing of a B. quintana isolate allowed us to propose a genome-wide sequence-based typing method. To ensure sequence discrimination based on highly polymorphic areas, we amplifie...
متن کاملMultispacer Sequence Typing for Mycobacterium tuberculosis Genotyping
BACKGROUND Genotyping methods developed to survey the transmission dynamics of Mycobacterium tuberculosis currently rely on the interpretation of restriction and amplification profiles. Multispacer sequence typing (MST) genotyping is based on the sequencing of several intergenic regions selected after complete genome sequence analysis. It has been applied to various pathogens, but not to M. tub...
متن کاملMolecular typing of Leptospira interrogans strains isolated from Rattus tanezumi in Guizhou Province, Southwest of China.
OBJECTIVE To identify and type three leptospires isolated from Rattus tanezumi in Guizhou Province by using three molecular techniques (PFGE, MLVA, and MLST), reveal the molecular characteristic of causative agents of local leptospirosis and evaluate these three molecular methods based on their detection resolution and efficiency. METHODS Three Leptospira strains were isolated from the kidney...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2013
ISSN: 0095-1137
DOI: 10.1128/jcm.02482-13